(CNN) - The Tea Party Express says it is going to target Alaska Sen. Lisa Murkowski - again.
The national Sacramento-based organization announced Thursday that next week they'll unveil a new television and radio ad campaign against Murkowski, who is running in the general election as a write-in candidate.
Last month, Murkowski conceded defeat in her bid for her party's nomination. The senator, who is part of a political dynasty in Alaska, was upset in the August 24 Republican Senate primary by attorney Joe Miller, a previous longshot who enjoyed strong support from Tea Party activists and former Alaska Gov. Sarah Palin.
The Tea Party Express had endorsed Miller and spent over $600,000 to help him defeat Murkowski.
"Lisa, take a hint, the voters rejected you once before and now watching you defy their will is bound to alienate yourself from Alaska voters even more," says Amy Kremer, chairwoman of the Tea Party Express, in a news release.
The group says it will hold a two-hour "No Means No" radio marathon on an Anchorage-based radio station in opposition to Murkowski's write-in campaign.
The move comes as Murkowski's campaign is up with new new commercials on Alaska television, one of which takes aim at the Tea Party Express.
"So they're going to come up here and drop another dirty money bomb on Alaska and try to take our seat," says one women in the ad.
"You know, they fooled a lot of people before the primary but we're on to them now," adds a man in the commercial.
A CNN/Time/Opinion Research Corporation survey released Wednesday indicates that 38 percent of likely voters in the state support Miller, while 36 percent back Murkowski. Twenty-two percent back Sitka Mayor Scott McAdams, the Democratic nominee, with four percent either undecided or not supporting any of the three candidates listed in the survey.
Miller's two-point advantage over Murkowski is within the survey's sampling error.
Follow Paul Steinhauser on Twitter: @PsteinhauserCNN